COUP-TF interacting protein 2 represses the initial phase of HIV-1 gene transcription in human microglial cells
Identifieur interne : 000186 ( France/Analysis ); précédent : 000185; suivant : 000187COUP-TF interacting protein 2 represses the initial phase of HIV-1 gene transcription in human microglial cells
Auteurs : Ce Line Marban [France] ; Laetitia Redel [France] ; Stella Suzanne [France] ; Carine Van Lint [Belgique] ; Dominique Lecestre [France] ; Sylvette Chasserot-Golaz [France] ; Mark Leid [France] ; Dominique Aunis [France] ; Evelyne Schaeffer [France] ; Olivier Rohr [France]Source :
- Nucleic Acids Research [ 0305-1048 ] ; 2005.
Abstract
Human immunodeficiency virus type 1 (HIV-1) gene transcription is characterized by two temporally distinct phases. While the initial phase relies solely on cellular transcription factors, the subsequent phase is activated by the viral Tat transactivator. We have previously reported that the subsequent phase of viral gene transcription can be repressed by the chicken ovalbumin upstream promoter transcription factor (COUP-TF)-interacting protein 2 (CTIP2) in human microglial cells [O. Rohr, D. Lecestre, S. Chasserot-Golaz, C. Marban, D. Avram, D. Aunis, M. Leid and E. Schaeffer (2003), J. Virol., 77, 5415–5427]. Here, we demonstrate that CTIP proteins also repress the initial phase of HIV-1 gene transcription, mainly supported by the cellular transcription factors Sp1 and COUP-TF in microglial cells. We report that CTIP2 represses Sp1- and COUP-TF-mediated activation of HIV-1 gene transcription and viral replication as a result of physical interactions with COUP-TF and Sp1 in microglial nuclei. Using laser confocal microscopy CTIP2 was found to colocalize with Sp1, COUP-TF and the heterochromatin-associated protein Hp1α, which is mainly detected in transcriptionally repressed heterochromatic region. Moreover, we describe that CTIP2 can be recruited to the HIV-1 promoter via its association with Sp1 bound to the GC-box sequences of the long terminal repeat (LTR). Since our findings demonstrate that CTIP2 interacts with the HIV-1 proximal promoter, it is likely that CTIP2 promotes HIV-1 gene silencing by forcing transcriptionally repressed heterochromatic environment to the viral LTR region.
Url:
DOI: 10.1093/nar/gki529
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000F16
- to stream Istex, to step Curation: 000F07
- to stream Istex, to step Checkpoint: 001246
- to stream Main, to step Merge: 001823
- to stream Main, to step Curation: 001814
- to stream Main, to step Exploration: 001814
- to stream France, to step Extraction: 000186
Links to Exploration step
ISTEX:25871AC55C443E416820C89D75F33BC275F44B2DLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">COUP-TF interacting protein 2 represses the initial phase of HIV-1 gene transcription in human microglial cells</title><author><name sortKey="Marban, Ce Line" sort="Marban, Ce Line" uniqKey="Marban C" first="Ce Line" last="Marban">Ce Line Marban</name></author><author><name sortKey="Redel, Laetitia" sort="Redel, Laetitia" uniqKey="Redel L" first="Laetitia" last="Redel">Laetitia Redel</name></author><author><name sortKey="Suzanne, Stella" sort="Suzanne, Stella" uniqKey="Suzanne S" first="Stella" last="Suzanne">Stella Suzanne</name></author><author><name sortKey="Van Lint, Carine" sort="Van Lint, Carine" uniqKey="Van Lint C" first="Carine" last="Van Lint">Carine Van Lint</name></author><author><name sortKey="Lecestre, Dominique" sort="Lecestre, Dominique" uniqKey="Lecestre D" first="Dominique" last="Lecestre">Dominique Lecestre</name></author><author><name sortKey="Chasserot Golaz, Sylvette" sort="Chasserot Golaz, Sylvette" uniqKey="Chasserot Golaz S" first="Sylvette" last="Chasserot-Golaz">Sylvette Chasserot-Golaz</name></author><author><name sortKey="Leid, Mark" sort="Leid, Mark" uniqKey="Leid M" first="Mark" last="Leid">Mark Leid</name></author><author><name sortKey="Aunis, Dominique" sort="Aunis, Dominique" uniqKey="Aunis D" first="Dominique" last="Aunis">Dominique Aunis</name></author><author><name sortKey="Schaeffer, Evelyne" sort="Schaeffer, Evelyne" uniqKey="Schaeffer E" first="Evelyne" last="Schaeffer">Evelyne Schaeffer</name></author><author><name sortKey="Rohr, Olivier" sort="Rohr, Olivier" uniqKey="Rohr O" first="Olivier" last="Rohr">Olivier Rohr</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:25871AC55C443E416820C89D75F33BC275F44B2D</idno><date when="2005" year="2005">2005</date><idno type="doi">10.1093/nar/gki529</idno><idno type="url">https://api.istex.fr/document/25871AC55C443E416820C89D75F33BC275F44B2D/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000F16</idno><idno type="wicri:Area/Istex/Curation">000F07</idno><idno type="wicri:Area/Istex/Checkpoint">001246</idno><idno type="wicri:doubleKey">0305-1048:2005:Marban C:coup:tf:interacting</idno><idno type="wicri:Area/Main/Merge">001823</idno><idno type="wicri:Area/Main/Curation">001814</idno><idno type="wicri:Area/Main/Exploration">001814</idno><idno type="wicri:Area/France/Extraction">000186</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">COUP-TF interacting protein 2 represses the initial phase of HIV-1 gene transcription in human microglial cells</title><author><name sortKey="Marban, Ce Line" sort="Marban, Ce Line" uniqKey="Marban C" first="Ce Line" last="Marban">Ce Line Marban</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM unité 575 Pathophysiology of Nervous System, Centre de Neurochimie 5 rue Blaise Pascal, 67084 Strasbourg</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Redel, Laetitia" sort="Redel, Laetitia" uniqKey="Redel L" first="Laetitia" last="Redel">Laetitia Redel</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM unité 575 Pathophysiology of Nervous System, Centre de Neurochimie 5 rue Blaise Pascal, 67084 Strasbourg</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Suzanne, Stella" sort="Suzanne, Stella" uniqKey="Suzanne S" first="Stella" last="Suzanne">Stella Suzanne</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM unité 575 Pathophysiology of Nervous System, Centre de Neurochimie 5 rue Blaise Pascal, 67084 Strasbourg</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Van Lint, Carine" sort="Van Lint, Carine" uniqKey="Van Lint C" first="Carine" last="Van Lint">Carine Van Lint</name><affiliation wicri:level="1"><country xml:lang="fr">Belgique</country><wicri:regionArea>Institute for Molecular Biology and Medicine, Laboratory of Molecular Virology 12 rue des Professeurs Jeener et Brachet, 6041 Gosselies</wicri:regionArea><wicri:noRegion>6041 Gosselies</wicri:noRegion></affiliation></author><author><name sortKey="Lecestre, Dominique" sort="Lecestre, Dominique" uniqKey="Lecestre D" first="Dominique" last="Lecestre">Dominique Lecestre</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM unité 575 Pathophysiology of Nervous System, Centre de Neurochimie 5 rue Blaise Pascal, 67084 Strasbourg</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Chasserot Golaz, Sylvette" sort="Chasserot Golaz, Sylvette" uniqKey="Chasserot Golaz S" first="Sylvette" last="Chasserot-Golaz">Sylvette Chasserot-Golaz</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Unité CNRS UPR 2356 5, rue Blaise Pascal, 67084 Strasbourg</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Leid, Mark" sort="Leid, Mark" uniqKey="Leid M" first="Mark" last="Leid">Mark Leid</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratory of Molecular Pharmacology, College of Pharmacy and Environmental Health Sciences Center, Oregon State University Corvallis, Oregon 97331-3507</wicri:regionArea><placeName><region type="region" nuts="2">Guyane</region></placeName></affiliation></author><author><name sortKey="Aunis, Dominique" sort="Aunis, Dominique" uniqKey="Aunis D" first="Dominique" last="Aunis">Dominique Aunis</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM unité 575 Pathophysiology of Nervous System, Centre de Neurochimie 5 rue Blaise Pascal, 67084 Strasbourg</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Schaeffer, Evelyne" sort="Schaeffer, Evelyne" uniqKey="Schaeffer E" first="Evelyne" last="Schaeffer">Evelyne Schaeffer</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM unité 575 Pathophysiology of Nervous System, Centre de Neurochimie 5 rue Blaise Pascal, 67084 Strasbourg</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Rohr, Olivier" sort="Rohr, Olivier" uniqKey="Rohr O" first="Olivier" last="Rohr">Olivier Rohr</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INSERM unité 575 Pathophysiology of Nervous System, Centre de Neurochimie 5 rue Blaise Pascal, 67084 Strasbourg</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>IUT Louis Pasteur de Schiltigheim, 1 Allée d'Athènes 67300 Schiltigheim</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Schiltigheim</settlement></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">France</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Nucleic Acids Research</title><title level="j" type="abbrev">Nucl. Acids Res.</title><idno type="ISSN">0305-1048</idno><idno type="eISSN">1362-4962</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2005">2005</date><biblScope unit="volume">33</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="2318">2318</biblScope><biblScope unit="page" to="2331">2331</biblScope></imprint><idno type="ISSN">0305-1048</idno></series><idno type="istex">25871AC55C443E416820C89D75F33BC275F44B2D</idno><idno type="DOI">10.1093/nar/gki529</idno><idno type="local">gki529</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0305-1048</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Human immunodeficiency virus type 1 (HIV-1) gene transcription is characterized by two temporally distinct phases. While the initial phase relies solely on cellular transcription factors, the subsequent phase is activated by the viral Tat transactivator. We have previously reported that the subsequent phase of viral gene transcription can be repressed by the chicken ovalbumin upstream promoter transcription factor (COUP-TF)-interacting protein 2 (CTIP2) in human microglial cells [O. Rohr, D. Lecestre, S. Chasserot-Golaz, C. Marban, D. Avram, D. Aunis, M. Leid and E. Schaeffer (2003), J. Virol., 77, 5415–5427]. Here, we demonstrate that CTIP proteins also repress the initial phase of HIV-1 gene transcription, mainly supported by the cellular transcription factors Sp1 and COUP-TF in microglial cells. We report that CTIP2 represses Sp1- and COUP-TF-mediated activation of HIV-1 gene transcription and viral replication as a result of physical interactions with COUP-TF and Sp1 in microglial nuclei. Using laser confocal microscopy CTIP2 was found to colocalize with Sp1, COUP-TF and the heterochromatin-associated protein Hp1α, which is mainly detected in transcriptionally repressed heterochromatic region. Moreover, we describe that CTIP2 can be recruited to the HIV-1 promoter via its association with Sp1 bound to the GC-box sequences of the long terminal repeat (LTR). Since our findings demonstrate that CTIP2 interacts with the HIV-1 proximal promoter, it is likely that CTIP2 promotes HIV-1 gene silencing by forcing transcriptionally repressed heterochromatic environment to the viral LTR region.</div></front></TEI><affiliations><list><country><li>Belgique</li><li>France</li></country><region><li>Alsace (région administrative)</li><li>Grand Est</li><li>Guyane</li></region><settlement><li>Schiltigheim</li><li>Strasbourg</li></settlement></list><tree><country name="France"><region name="Grand Est"><name sortKey="Marban, Ce Line" sort="Marban, Ce Line" uniqKey="Marban C" first="Ce Line" last="Marban">Ce Line Marban</name></region><name sortKey="Aunis, Dominique" sort="Aunis, Dominique" uniqKey="Aunis D" first="Dominique" last="Aunis">Dominique Aunis</name><name sortKey="Chasserot Golaz, Sylvette" sort="Chasserot Golaz, Sylvette" uniqKey="Chasserot Golaz S" first="Sylvette" last="Chasserot-Golaz">Sylvette Chasserot-Golaz</name><name sortKey="Lecestre, Dominique" sort="Lecestre, Dominique" uniqKey="Lecestre D" first="Dominique" last="Lecestre">Dominique Lecestre</name><name sortKey="Leid, Mark" sort="Leid, Mark" uniqKey="Leid M" first="Mark" last="Leid">Mark Leid</name><name sortKey="Redel, Laetitia" sort="Redel, Laetitia" uniqKey="Redel L" first="Laetitia" last="Redel">Laetitia Redel</name><name sortKey="Rohr, Olivier" sort="Rohr, Olivier" uniqKey="Rohr O" first="Olivier" last="Rohr">Olivier Rohr</name><name sortKey="Rohr, Olivier" sort="Rohr, Olivier" uniqKey="Rohr O" first="Olivier" last="Rohr">Olivier Rohr</name><name sortKey="Rohr, Olivier" sort="Rohr, Olivier" uniqKey="Rohr O" first="Olivier" last="Rohr">Olivier Rohr</name><name sortKey="Schaeffer, Evelyne" sort="Schaeffer, Evelyne" uniqKey="Schaeffer E" first="Evelyne" last="Schaeffer">Evelyne Schaeffer</name><name sortKey="Suzanne, Stella" sort="Suzanne, Stella" uniqKey="Suzanne S" first="Stella" last="Suzanne">Stella Suzanne</name></country><country name="Belgique"><noRegion><name sortKey="Van Lint, Carine" sort="Van Lint, Carine" uniqKey="Van Lint C" first="Carine" last="Van Lint">Carine Van Lint</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Belgique/explor/OpenAccessBelV2/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000186 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000186 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Belgique
|area= OpenAccessBelV2
|flux= France
|étape= Analysis
|type= RBID
|clé= ISTEX:25871AC55C443E416820C89D75F33BC275F44B2D
|texte= COUP-TF interacting protein 2 represses the initial phase of HIV-1 gene transcription in human microglial cells
}}
| This area was generated with Dilib version V0.6.25. |  |